This insightful research was conducted by three Egyptian Microbiologists; Samar Solyman, Mohamed Reda and Amro Hanora of the Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Egypt.
The need for a Staphylococcus aureus vaccine cannot be over emphasized due to its super-bug nature towards a number of common antibiotics. In the researchers own words; Staphylococcus aureus (S. aureus) is a versatile bacterium which exhibits multiple antibiotic resistances. To ameliorate the undesirable diseases causing potential, there is a need to design a protective vaccine capable of stimulating immune response against this pathogen.
The researchers reported that in a similar study in their laboratory, reverse vaccinology approach was used to nominate potential vaccine candidate genes against S. aureus. Zinc Metalloproteinase Aureolysin (aur) gene was one of the nominated genes based on that previously published in-silico study.
Thus, as a follow-up study, the objective of the current study was the cloning, expression, purification of “aur” gene and testing the “aur” protein reactivity with serum antibodies collected from groups of human patients with confirmed Staphylococcal disease.
During the study, cloning was done in pH6HTN His6HaloTag® vector and expressed in E. coli BL21 (DE3) using these conditions; 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 h at 37°C. Purification was carried out by Immobilized Metal Affinity Chromatography (IMAC).
The result of the study showed that the his-tag aur protein was detected at ~86 KDa as a single band after western blot assay and was successfully reacted with antibodies obtained from humans infected with S. aureus. At the end of the study, the researchers concluded that the study results encouraged further testing of aur protein as a potential vaccine candidate for S. aureus.