Statistical optimization of L–glutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrate

A Research Agenda on the Sexual and Impact of Statistical optimization of L–glutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrateTEGO® hybrid maize variety on agricultural productivity and poverty alleviation in KenyaHealth Dimensions of the COVID-19 Pandemic in Africa
A Research Agenda on the Sexual and Impact of Statistical optimization of L–glutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrateTEGO® hybrid maize variety on agricultural productivity and poverty alleviation in KenyaHealth Dimensions of the COVID-19 Pandemic in Africa

L-glutaminase is an amidase enzyme that converts glutamine to glutamate. Glutamate is the anion of glutamic acid, a chemical that nerve cells use to send signals to other cells. It is by a wide margin the most abundant excitatory neurotransmitter in the vertebrate nervous system.

Recently, Musa Olatunji Olarewaju of the Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Nigeria examined six strains of Trichoderma species isolated from soil and air samples were screened for extracellular L-glutaminase production.

His highest enzyme producing isolate, Trichoderma longibrachiatum was subjected to screening and nutrients optimization experiments under solid state fermentation using a constant weight of African locust beans as substrate. In his research methods, specific activity of 54.96 U/mg was achieved at 1% (w/v) glutamine, 0.5% (w/v) glucose, 1% (w/v) NaCl, pH 3.0 and 1.4 ml inoculums size respectively after 3 days of incubation at 27°C as the optimum condition for L–glutaminase production.

He observed that aside glutamine, other nitrogen sources; malt extract and ammonium sulphate had a negative influence on enzyme production likewise the supplemented sources of metal ions; zinc and iron (II) ions.

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